Phosphate Buffered Saline aka PBS

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Phosphate Buffered Saline aka PBS
Dissolve the following in 800 ml MQ H₂O.
8 g NaCl
0.2 g KCl
1.44 g Na₂HPO₄
0.24 g KH₂PO₄
Adjust the pH.
Add MQ H₂O to 1L.
Sterilize to autoclave.
Store at RT.
Make 1L of PBS pH 7 and 1L PBS pH 8, then mix accordingly to obtain desired pH.
For soils, will want PBS at pH ~7.4.
300 ml PBS pH 7 + 200 ml PBS pH 8 = 0.5 L PBS pH 7.4.

“PBS”缓冲液

PBS的制备与消毒(ppt 下载)

Phosphate Buffered Saline aka PBS

常用贮存液的配制之磷酸盐缓冲溶液（PBS）溶液的

Phosphate buffered saline(磷酸盐缓冲液)