Phosphate buffered saline(磷酸盐缓冲液)

- Dissolve in 800 ml of distilled H₂O:
- 8 g of NaCl
- 0.2 g of KCl
- 1.44 g of Na₂HPO₄
- 0.27 g of KH₂PO₄
- Adjust the pH to 7.4 with HCl or NaOH
- Add H₂O to 1 liter.

Dispense the solution into aliquots and sterilize them by autoclaving (20 min, 121°C, liquid cycle). Store at room temperature.

Phosphate buffered saline (abbreviated PBS) is a buffer solution commonly used in biological research. It is a salty solution containing sodium chloride, sodium phosphate, and (in some formulations) potassium chloride and potassium phosphate. The buffer helps to maintain a constant pH. The osmolarity and ion concentrations of the solution usually match those of the human body (isotonic).

Applications
PBS has many uses because it is isotonic and non-toxic to cells. It can be used to dilute substances. It is used to rinse containers containing cells. PBS can be used as a diluent in methods to dry biomolecules, as water molecules within it will be structured around the substance (protein, for example) to be 'dried' and immobilized to a solid surface[citation needed]. The thin film of water that binds to the substance prevents denaturation or other conformational changes. Carbonate buffers may be used for the same purpose but with less effectiveness[citation needed]. PBS can be used to take a reference spectrum when measuring the protein adsorption in ellipsometry[citation needed].