National Committee for Clinical Laboratory Standards. 2003. Methods for dilution antimicrobial susceptibility tests for bacteria thatgrow aerobically,5th ed. Approved standard M7–A6. National Committ....
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End repair: Add 5-10 units of T4 DNApol and incubate at 37C for 5 minutes.
Make solution to 2.5 M NH4OAc and add 1 volume of 95% ethanol; let sit for 5 minutes at RT and spin at 14K x g for 20 minut....
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When designing PCR experiments in which the synthesized DNA fragment is to be subsequently digested with a RE, it is very important to determine how many extra nucleotides should be added to the 5’-e....
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Cleavage of PCR Products Directly After Amplification Reaction
In experiments with amplified DNA, restriction endonuclease (RE) digestion is usually performed directly in the PCR mixture, without tim....
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Place the sephadex measuring plate (MultiScreen ?Column Loader) on a clean piece of saran wrap. Pour some sephadex onto the plate (takes about 3.3 g). Scrape the surface with the Multiscreen metal pla....
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Cloning PCR products using TA vectorsby Paul N. Hengen, Ph.D. *Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds-reagnts, availa....
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PEG PRECIPITATION OF PCR PRODUCTS
This protocol can be used instead of EXO/SAP for removing excess primers and nucleotides from PCR products before cycle-sequencing. Protocol:1. After PCR, add the fo....
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