惠静毅——中科院上海生命科学研究院生化与细胞所

研究员，研究组长，博士生导师
单位： 中科院上海生命科学研究院生化与细胞所
电话：021-54921354
E-mail: jyhui@sibs.ac.cn
         1993年复旦大学微生物系毕业，获学士学位；1999年德国Free University Berlin毕业，获硕士学位；2003年德国Justus-Liebig-University Giessen毕业，获博士学位。2003至2008年，先后在德国Justus-Liebig-University Giessen和Friedrich-Alexander-University Erlangen-Nuremberg从事博士后研究工。2008年起任中科院上海生科院生化与细胞所研究员，研究组长。
研究方向

哺乳动物细胞mRNA剪接的调控与功能
研究工作

      在哺乳动物细胞中，绝大多数蛋白编码基因含有外显子(exon)和内含子(intron)顺序。通常，内含子通过mRNA剪接(splicing)的过程从mRNA前体(pre-mRNA)中被精确和有效地去除；外显子则被剪接加工成为成熟的mRNA。然而，有一部分外显子通过可变剪接(alternative splicing)过程以不同方式生成mRNA异构体(isoforms)。它们往往能够编码具有不同、甚至带有拮抗功能的蛋白质。可变剪接具有组织、发育阶段及疾病进程特异性，并且受外界刺激地调控。因此，可变剪接是一个能够产生蛋白多样性及调控基因表达的重要机制。本研究组将采用生物化学，分子生物学，细胞生物学及生物信息学的手段探索在哺乳动物细胞基因表达过程中mRNA剪接的调控与功能。具体研究内容包括：
        (1)剪接调控蛋白调控mRNA剪接的机制；
        (2)利用生物信息学与生物芯片的手段寻找剪接调控蛋白的靶基因；
        (3)信号转导通路诱导可变剪接的机制与功能；
        (4)microRNA在剪接调控中的功能。
发表论文

1.Hung L.H.*, Heiner M.*, Hui J.*, Schreiner S., Benes V., and Bindereif A. Diverse roles of hnRNP L in mammalian mRNA processing: a combined microarray and RNAi analysis. RNA. 14, 284-296 (2008). banma@vip.qq.com
2.Lorenz M., Hewing B., Hui J., Zepp A., Baumann G., Bindereif A., Stangl V., Stangl K. Alternative splicing in intron 13 of the human eNOS gene: a potential mechanism for regulating eNOS activity. FASEB J. 21, 1556-1564 (2007). banma@vip.qq.com
3.Hui J., and Bindereif A. Alternative pre-mRNA splicing in the human system: unexpected role of repetitive sequences as regulatory elements. Biol Chem. 386, 1265-1271 (2005) (an invited review). banma@vip.qq.com
4.Hui J.*, Hung L.H.*, Heiner M., Schreiner S., Neumuller N., Reither G., Haas S.A., and Bindereif A. Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24, 1988-1998 (2005). banma@vip.qq.com
5.Palfi Z., Hui J., and Bindereif A. Biotin-based affinity purification of RNA-protein complexes. In Handbook of RNA biochemistry, (Westhof E., Bindereif A., Schön A., and Hartmann R.K., eds.), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, pp676-693 (2004). banma@vip.qq.com
6.Hui J., Reither G., and Bindereif A. Novel function role of CA repeats and hnRNP L in RNA stability. RNA. 9, 931-936 (2003). banma@vip.qq.com
7.Hui J., Stangl K., Lane W.L., and Bindereif A. HnRNP L stimulates splicing of the eNOS gene by binding to variable-length CA repeats. Nat. Struct. Biol. 10, 33-37 (2003). Comment by Bilbao D. and Valcárcel J. Getting to the heart of a splicing enhancer. Nat Struct Biol. 10, 6-7 (2003). banma@vip.qq.com
8.Yang J., Hui J., Li G., Wang Y., Yuan H., and Li Y. Expression of the recombinant hepatitis B virus surface antigen carrying preS epitopes in Pichia pastoris. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai), 32, 39-144 (2000). banma@vip.qq.com
9.Hui J., Li G., Kong Y., and Wang Y. Expression and characterization of chimeric hepatitis B surface antigen particles carrying preS epitopes. J. Biotechnol. 72, 49-59 (1999). banma@vip.qq.com
10.Hui J., Mancini M., Li G., Wang Y., Tiollais P., and Michel M.L. Immunization with a plasmid encoding a modified hepatitis B surface antigen carrying the receptor binding site for hepatocytes. Vaccine, 17, 1711-1718 (1999). banma@vip.qq.com
11.Hui J., Li G., Kong Y., and Wang Y. DNA-mediated immunization against hepatitis B surface antigen carrying preS epitopes. Chinese Science Bulletin, 44, 620-623 (1999). banma@vip.qq.com
12.Hui J., Li G., Kong Y., and Wang Y. A modified HBsAg containing the preS1 and preS2 epitopes. Science in China, (Series C), 41, 56-63 (1998).
(* equal contribution)banma@vip.qq.com